| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 691 | Methanosphaera cuniculi | GGAACAGGAGGACCAGGTT | TCGCCTGGTCTGATTGCA | 58.54 | 58.93 | 218 | 44 |
100.00%
|
100.00%
|
| Primer 692 | Methanosphaera cuniculi | TGGAAGCGGACCACTACCT | CGCGTTCCCATACATTTGCA | 60.23 | 59.55 | 251 | 44 |
100.00%
|
100.00%
|
| Primer 693 | Methanosphaera cuniculi | TGGAAGCGGACCACTACCT | ACGCGTTCCCATACATTTGC | 60.23 | 59.55 | 252 | 44 |
100.00%
|
100.00%
|
| Primer 694 | Methanosphaera cuniculi | CTCCTGCACCACCAGAGTT | GAGGACGTCTAGCCCAAAGT | 59.24 | 59.10 | 270 | 44 |
100.00%
|
100.00%
|
| Primer 695 | Methanosphaera cuniculi | CTCCTGCACCACCAGAGTT | AGGACGTCTAGCCCAAAGTTG | 59.24 | 60.00 | 269 | 44 |
100.00%
|
100.00%
|
| Primer 696 | Methanosphaera cuniculi | GCATGGGCACAAGTTGATGTA | TCAGCAACACCCTGTGCA | 59.18 | 59.41 | 155 | 44 |
100.00%
|
100.00%
|
| Primer 697 | Methanosphaera cuniculi | TGTGACGACTGGGGAATGC | TGCTACTCCTGCTCCACCT | 60.00 | 59.92 | 285 | 44 |
100.00%
|
100.00%
|
| Primer 698 | Methanosphaera cuniculi | ACCACAAAGAAAAGTACGCCT | TCGTCCTCCTACTCCTTGGA | 58.07 | 59.00 | 166 | 44 |
100.00%
|
100.00%
|
| Primer 699 | Methanosphaera cuniculi | ACCACAAAGAAAAGTACGCCT | AGTGCTGATACTCGTCCTCCT | 58.07 | 60.06 | 177 | 44 |
100.00%
|
100.00%
|
| Primer 700 | Methanosphaera cuniculi | TGACTTCATGGGAGCACGAG | CCTTCACGTATCCGGTAGTCA | 59.75 | 58.98 | 186 | 43 |
100.00%
|
100.00%
|